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sd trp medium  (TaKaRa)


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    Structured Review

    TaKaRa sd trp medium
    Sd Trp Medium, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sd+trp+medium/SD%2F-Trp+Broth/10__1093_slash_hr_slash_uhag157-209-19-23
    Average 94 stars, based on 10 article reviews
    sd trp medium - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: The HD-ZIP I transcription factor BcLMI1 regulates bolting time in <i>Brassica campestris</i> by repressing <i>BcTCP4</i>
    Article Snippet: Yeast one-hybrid assays were performed using 433 the MATCHMAKER system (Clontech, Mountain View, CA, USA) according to the 434 AC CE PT ED M AN US CR IP T D ow nloaded from https://academ ic.oup.com /hr/advance-article/doi/10.1093/hr/uhag157/8658096 by guest on 01 M ay 2026 manufacturer’s protocol. .. Bait plasmids were transformed into Saccharomyces 435 cerevisiae strain EGY48 (Cat#: YC1030, WeiDi, Shanghai, China) and selected on 436 SD/-Trp medium (Cat#: 630308, Clontech, USA). .. Protein-DNA interaction was 437 determined based on the growth ability of the co-transformed yeast cells on the SD/-438 Trp/-Ura (DO Supplement-Trp/-Ura, CAT#: PM2260, Coolaber, Beijing, China) 439 dropout medium containing X-gal (X-gal CAT#: CX11921, Coolaber, Beijing, China) 440 at 30°C.

    Article Title: PIF8-WRKY42-mediated salicylic acid synthesis modulates red light induced powdery mildew resistance in oriental melon.
    Article Snippet: Funding information China Agriculture Research System of MOF and MARA; National Natural Science Foundation of China Abstract Light signals and plant hormones are involved in regulating the growth, development and stress resistance of plants; however, it remains unclear whether light affects hormones and thus pathogen resistance in oriental melon.. Here, we found that red light promoted salicylic acid (SA) accumulation and powdery mildew resistance by activating the transcription of CmICS, the key gene for SA biosynthesis, and silencing CmICS seriously weakened the induction effect of red light on powdery mildew resistance in oriental melon leaves.. Further studies showed that red light induced the expression of CmWRKY42 under powdery mildew stress, and CmWRKY42 directly bound to the CmICS promoter to activate its expression and promote the accumulation of SA under red light.

    Cell Culture:

    Article Title: Two sides of the coin.
    Article Snippet: .. Nature GeNetics doi:10.1038/ng.2465 colonies were cultured in SD-Trp– medium overnight and were grown in SD-Gal-Raff induction medium (Clontech) for 12 h before quantification. .. Yeast two-hybrid screening was performed by Hybrigenics Services (Paris, France) (see URLs). pB27-TMF was used as bait to screen a random-primed tomato cDNA library (vegetative meristems, reproductive meristems, P3–P5 leaf primordia with stems and floral buds) constructed in pP6 plasmid. pB27 and pP6 derive from the original pBTM116 plasmid57 and pGADGH58 plasmids, respectively.

    Article Title: PIF8-WRKY42-mediated salicylic acid synthesis modulates red light induced powdery mildew resistance in oriental melon.
    Article Snippet: Funding information China Agriculture Research System of MOF and MARA; National Natural Science Foundation of China Abstract Light signals and plant hormones are involved in regulating the growth, development and stress resistance of plants; however, it remains unclear whether light affects hormones and thus pathogen resistance in oriental melon.. Here, we found that red light promoted salicylic acid (SA) accumulation and powdery mildew resistance by activating the transcription of CmICS, the key gene for SA biosynthesis, and silencing CmICS seriously weakened the induction effect of red light on powdery mildew resistance in oriental melon leaves.. Further studies showed that red light induced the expression of CmWRKY42 under powdery mildew stress, and CmWRKY42 directly bound to the CmICS promoter to activate its expression and promote the accumulation of SA under red light.

    Article Title: HPE21, a liberibacter effector modulating H2O2 accumulation in tomato plants
    Article Snippet: L. solanacearum' B-infected psyllids by PCR as previously described and cloned into pDONR207 vector by Gateway BP Clonase II (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's directions. pDONR207:HPE13 and pDONR207:HPE21 were used to subclone into the pDEST-GBKT7 vector with the Gateway LR Clonase II enzyme (Thermo Fisher Scientific) and transformed into the Y2H Gold strain (Takara Bio, San Jose, CA, USA) for Y2H screening as a bait. .. HPE13 and HPE21 bait strains were cultured separately in SD-Trp medium (Takara Bio, #630309) and brought to a final concentration of > 108 cells/mL. ..

    Y1H Assay:

    Article Title: PIF8-WRKY42-mediated salicylic acid synthesis modulates red light induced powdery mildew resistance in oriental melon.
    Article Snippet: Funding information China Agriculture Research System of MOF and MARA; National Natural Science Foundation of China Abstract Light signals and plant hormones are involved in regulating the growth, development and stress resistance of plants; however, it remains unclear whether light affects hormones and thus pathogen resistance in oriental melon.. Here, we found that red light promoted salicylic acid (SA) accumulation and powdery mildew resistance by activating the transcription of CmICS, the key gene for SA biosynthesis, and silencing CmICS seriously weakened the induction effect of red light on powdery mildew resistance in oriental melon leaves.. Further studies showed that red light induced the expression of CmWRKY42 under powdery mildew stress, and CmWRKY42 directly bound to the CmICS promoter to activate its expression and promote the accumulation of SA under red light.

    Concentration Assay:

    Article Title: HPE21, a liberibacter effector modulating H2O2 accumulation in tomato plants
    Article Snippet: L. solanacearum' B-infected psyllids by PCR as previously described and cloned into pDONR207 vector by Gateway BP Clonase II (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's directions. pDONR207:HPE13 and pDONR207:HPE21 were used to subclone into the pDEST-GBKT7 vector with the Gateway LR Clonase II enzyme (Thermo Fisher Scientific) and transformed into the Y2H Gold strain (Takara Bio, San Jose, CA, USA) for Y2H screening as a bait. .. HPE13 and HPE21 bait strains were cultured separately in SD-Trp medium (Takara Bio, #630309) and brought to a final concentration of > 108 cells/mL. ..



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